國家衛生研究院 NHRI:Item 3990099045/2570

NHRI > National Institute of Environmental Health Sciences > Dennis P.H. Hsieh(2004-2007) > Periodical Articles > Item 3990099045/2570

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2570

Title:	Liquid chromatography/tandem mass spectrometric method for the simultaneous determination of tobacco-specific nitrosamine NNK and its five metabolites
Authors:	Lee, HL;Wang, CY;Lin, S;Hsieh, DPH
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	A sensitive and robust high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method to analyze 4(methylnitrosamino)-1-(3-pyridyl)-l-butanone (NNK) and its five metabolites in one passage was developed and validated. The method achieved excellent reproducibility and accuracy. Linearity was observed for all six compounds (R-2 = 0.999) with detection limits (S/N >= 3) ranging from 0.2 to 2.4pg on column and 0.01-0.12ngm(-l) in samples injected. Average intra-day and inter-day variations (% R.S.D.) were 1.2 and 3.5%, respectively. A sample preparation method involving C8 and C18 solid phase extraction provided satisfactory recovery of the analytes in mouse urine. Each NNK metabolite was identified by its chromatographic retention time and specific fragmentation pattern. Since the carcinogenicity of NNK is related to its metabolism, the method described in this report should facilitate toxicological investigations into the carcinogenesis due to NNK exposure in the environment. (c) 2007 Elsevier B.V. All rights reserved.
Keywords:	Chemistry, Analytical
Date:	2007-08-15
Relation:	Talanta. 2007 Aug;73(1):76-80.
Link to:	http://dx.doi.org/10.1016/j.talanta.2007.03.001
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0039-9140&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000249174700010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34547136468
Appears in Collections:	[Dennis P.H. Hsieh(2004-2007)] Periodical Articles

Files in This Item:

File	Description	Size	Format
000249174700010.pdf		149Kb	Adobe PDF	1189	View/Open

Loading...