國家衛生研究院 NHRI:Item 3990099045/9442

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Title:	Fabrication of keratin/fibroin membranes by electrospinning for vascular tissue engineering
Authors:	Yen, KC;Chen, CY;Huang, JY;Kuo, WT;Lin, FH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Cardiovascular diseases (CVDs) are some of the leading causes of death and bypass surgery is one of the common treatment options for the critical CVD patients. There is no ideal material available for arterial bypass surgery. Herein, a fibroin and keratin porous membrane was prepared by electro-spinning and proposed for tissue-engineered vascular grafts (TEVGs). The purified fibroin (F) and keratin (K) were mixed in different weight ratios of 9 : 1 (FK91), 8 : 2 (FK82), and 7 : 3 (FK73) to obtain a membrane. The SEM images revealed that the electro-spinned membranes have a fibrous interconnected porous structure. The average diameter of the membrane F, FK91, FK82 and FK73 was 5.74 +/- 1.04, 4.20 +/- 1.19, 2.94 +/- 0.81 and 2.27 +/- 0.65 [small mu ]m, respectively. The ultimate tensile strength (UTS) of F, FK91, FK82 and FK73 was 2.09 +/- 0.06, 2.02 +/- 0.06, 1.81 +/- 0.10 and 1.74 +/- 0.12 MPa, respectively. The contact angle of F, FK91, FK82 and FK73 was 72.55 +/- 0.55[degree], 66.39 +/- 0.90[degree], 43.47 +/- 0.04[degree] and 33.65 +/- 2.83[degree], respectively. The wettability results were in agreement with those of the cell adhesion to the electro-spinning membranes. The attached HUVECs on the developed membranes showed no cytotoxicity. The immunocytochemistry staining and qPCR analysis showed that the phenotype of the keratin-fibroin membrane was not altered. The results of the ultimate tensile strength, cell adhesion and microstructure revealed that FK82 is similar to native vessels and could be considered as a potential material for TEVGs.
Date:	2016-01-14
Relation:	Journal of Materials Chemistry B. 2016 Jan 14;4(2):237-244.
Link to:	http://dx.doi.org/10.1039/C5TB01921D
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2050-750X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000367335200008
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84952319172
Appears in Collections:	[Feng-Huei Lin] Periodical Articles

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