國家衛生研究院 NHRI:Item 3990099045/8018

NHRI > Institute of Molecular and Genomic Medicine > Chun-Hong Chen > Conference Papers/Meeting Abstract > Item 3990099045/8018

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8018

Title:	Dengue 2 virus infection activates autophagy that is favorable for viral replication in mosquito aedes aegypti
Authors:	Chen, CC;Tsao, IY;Lu, YC;Shiao, SH;Chen, CH;Tsai, CH
Contributors:	Institute of Molecular and Genomic Medicine
Abstract:	Autophagy is a catabolic process for the maintenance of cellular homeostasis and is also important in times of stress, including viral infection. Recently we found knockdown of three authophagy genes, ATG5, ATG8 and ATG18, significantly reduced the transcriptional levels and tiers of dengue 2 virus(DENV2) in Ades aegypti. We also found that DENV2 transcription level was significantly reduced after autophagy was blocked by 3 methyladenine or L-asparagine and was significantly enhanced after authophagy was induced by rapamycin. Western blotting analysis also demonstrated that level of the LC3-II, the widely used biomarker of authophagy, was significantly increased in DENV2-infected mosquitoes at 24h post inoculatin(p.i.) and reached the maximum at 48h p.i. Then is gradually declined but remained detectable at 96h p.i. Subsequently, our results suggested that in vector mosquitoes, DENV2 can activate autophagy machinery that is favorable for viral replication.
Date:	2013-12
Relation:	Pathogens and Global Health. 2013 Dec;107(8):404.
Link to:	http://dx.doi.org/10.1179/2047772413Z.000000000160
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2047-7724&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000335056200015
Appears in Collections:	[Chun-Hong Chen] Conference Papers/Meeting Abstract

Files in This Item:

File	Description	Size	Format
ISI000335056200015.pdf		111Kb	Adobe PDF	631	View/Open

Loading...