國家衛生研究院 NHRI:Item 3990099045/7293
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 857846      線上人數 : 838
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7293


    題名: Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection
    作者: Lin, HY;Yang, YT;Yu, SL;Hsiao, KN;Liu, CC;Sia, C;Chow, YH
    貢獻者: Division of Vaccine Research and Development
    摘要: Enterovirus 71 (EV71) causes hand, foot, and, mouth disease and severe neurological disorders in children. Human scavenger receptor class B member 2 (hSCARB2) and P-selectin glycoprotein ligand-1 (PSGL-1) are identified as receptors for EV71. The underling mechanism of PSGL-1-mediated EV71 entry remains unclear. The endocytosis required for EV71 entry were investigated in Jurkat T and mouse L929 cells constitutively expressing human PSGL-1 (PSGL-1-L929) or human rhabdomyosarcoma (RD) cells displaying high SCARB2 but no PSGL-1 by treatment of specific inhibitors or siRNA. We found that disruption of clathrin-dependent endocytosis prevented EV71 infection in RD cells while there was no influence in Jurkat T and PSGL-1-L929 cells. Disturbing caveolar endocytosis by specific inhibitor or caveolin-1 siRNA in Jurkat T and PSGL-1-L929 cells significantly blocked EV71 infection, while it had no effect on EV71 infection in RD cells. Confocal immunofluorescence demonstrated caveolea and EV71 was directly co-localizated. pH-dependent endosomal acidification and intact membrane cholesterol were important for EV71 infection as judged by the pretreatment of inhibitors which abrogated the infection. A receptor-dominated endocytosis of EV71 infection was observed: PSGL-1 initiates caveolae-dependent endocytosis and hSCARB2 activates clathrin-dependent endocytosis.
    日期: 2013-08
    關聯: Journal of Virology. 2013 Aug;7(16):9064-9076.
    Link to: http://dx.doi.org/10.1128/jvi.00573-13
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-538X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000322535600023
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84881227572
    顯示於類別:[周彥宏] 期刊論文
    [謝鐸源(2006-2014)] 期刊論文
    [劉家齊] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB23760234.pdf2971KbAdobe PDF652檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋