國家衛生研究院 NHRI:Item 3990099045/7293
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    题名: Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection
    作者: Lin, HY;Yang, YT;Yu, SL;Hsiao, KN;Liu, CC;Sia, C;Chow, YH
    贡献者: Division of Vaccine Research and Development
    摘要: Enterovirus 71 (EV71) causes hand, foot, and, mouth disease and severe neurological disorders in children. Human scavenger receptor class B member 2 (hSCARB2) and P-selectin glycoprotein ligand-1 (PSGL-1) are identified as receptors for EV71. The underling mechanism of PSGL-1-mediated EV71 entry remains unclear. The endocytosis required for EV71 entry were investigated in Jurkat T and mouse L929 cells constitutively expressing human PSGL-1 (PSGL-1-L929) or human rhabdomyosarcoma (RD) cells displaying high SCARB2 but no PSGL-1 by treatment of specific inhibitors or siRNA. We found that disruption of clathrin-dependent endocytosis prevented EV71 infection in RD cells while there was no influence in Jurkat T and PSGL-1-L929 cells. Disturbing caveolar endocytosis by specific inhibitor or caveolin-1 siRNA in Jurkat T and PSGL-1-L929 cells significantly blocked EV71 infection, while it had no effect on EV71 infection in RD cells. Confocal immunofluorescence demonstrated caveolea and EV71 was directly co-localizated. pH-dependent endosomal acidification and intact membrane cholesterol were important for EV71 infection as judged by the pretreatment of inhibitors which abrogated the infection. A receptor-dominated endocytosis of EV71 infection was observed: PSGL-1 initiates caveolae-dependent endocytosis and hSCARB2 activates clathrin-dependent endocytosis.
    日期: 2013-08
    關聯: Journal of Virology. 2013 Aug;7(16):9064-9076.
    Link to: http://dx.doi.org/10.1128/jvi.00573-13
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-538X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000322535600023
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84881227572
    显示于类别:[周彥宏] 期刊論文
    [謝鐸源(2006-2014)] 期刊論文
    [劉家齊] 期刊論文

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