國家衛生研究院 NHRI:Item 3990099045/3773
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    题名: Acrylamide-induced astrogliotic and apoptotic responses in human astrocytoma cells
    作者: Chen, JH;Wu, KY;Chiu, IM;Tsou, TC;Chou, CC
    贡献者: Division of Environmental Health and Occupational Medicine;Institute of Cellular and Systems Medicine
    摘要: This study was to clarify whether acrylamide (ACR) will induce apoptosis and astrogliosis in an astrocytic cell line in vitro. Different time- and dose-dependent cytotoxic studies were conducted upon neuronal (SH-SY5Y) and glial cell lines (U-1240 MG) under exposure to ACR up to 72 h. We showed that SH-SY5Y cells were more sensitive in cytotoxic assays than U-1240 MG cells, and significantly decreased cell viability was observed at concentrations higher than 1 mM with increased lactate dehydrogenase leakage observed only at 5 and 10 mM in U-1240 MG cells. The ACR-induced apoptotic responses and phosphorylation of p53 protein at Ser15 for U-1240 MG cells were identified at 48 h. The increase of glial fibrillary acidic protein (GFAP) as a chemical-induced astrogliotic response was found to be associated with different ACR concentrations and exposure times, particularly at ≧48 h of ≧2 mM. In addition, immunocytochemical staining at 36 h of 5 and 10 mM treatments had significantly higher density of GFAP than the control. Thus, ACR-induced effects can be seen in neuronal and astrocytic cells. These results suggest that ACR exposure may lead to apoptotic and astrogliotic effects in human astrocytoma cells in vitro in a time- and dose-dependent manner.
    日期: 2009-08
    關聯: Toxicology in Vitro. 2009 Aug;23(5):855-861.
    Link to: http://dx.doi.org/10.1016/j.tiv.2009.04.015
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0887-2333&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000268345500012
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67649494327
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