國家衛生研究院 NHRI:Item 3990099045/3773

NHRI > National Institute of Environmental Health Sciences > Tsui-Chun Tsou > Periodical Articles > Item 3990099045/3773

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/3773

Title:	Acrylamide-induced astrogliotic and apoptotic responses in human astrocytoma cells
Authors:	Chen, JH;Wu, KY;Chiu, IM;Tsou, TC;Chou, CC
Contributors:	Division of Environmental Health and Occupational Medicine;Institute of Cellular and Systems Medicine
Abstract:	This study was to clarify whether acrylamide (ACR) will induce apoptosis and astrogliosis in an astrocytic cell line in vitro. Different time- and dose-dependent cytotoxic studies were conducted upon neuronal (SH-SY5Y) and glial cell lines (U-1240 MG) under exposure to ACR up to 72 h. We showed that SH-SY5Y cells were more sensitive in cytotoxic assays than U-1240 MG cells, and significantly decreased cell viability was observed at concentrations higher than 1 mM with increased lactate dehydrogenase leakage observed only at 5 and 10 mM in U-1240 MG cells. The ACR-induced apoptotic responses and phosphorylation of p53 protein at Ser15 for U-1240 MG cells were identified at 48 h. The increase of glial fibrillary acidic protein (GFAP) as a chemical-induced astrogliotic response was found to be associated with different ACR concentrations and exposure times, particularly at ≧48 h of ≧2 mM. In addition, immunocytochemical staining at 36 h of 5 and 10 mM treatments had significantly higher density of GFAP than the control. Thus, ACR-induced effects can be seen in neuronal and astrocytic cells. These results suggest that ACR exposure may lead to apoptotic and astrogliotic effects in human astrocytoma cells in vitro in a time- and dose-dependent manner.
Date:	2009-08
Relation:	Toxicology in Vitro. 2009 Aug;23(5):855-861.
Link to:	http://dx.doi.org/10.1016/j.tiv.2009.04.015
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0887-2333&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000268345500012
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67649494327
Appears in Collections:	[Tsui-Chun Tsou] Periodical Articles [Ing-Ming Chiu] Periodical Articles

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