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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2952


    Title: In vitro hepatic differentiation of human mesenchymal stem cells
    Authors: Lee, KD;Kuo, TKC;Whang-Peng, J;Chung, YF;Lin, CT;Chou, SH;Chen, JR;Chen, YP;Lee, OKS
    Contributors: National Institute of Cancer Research
    Abstract: This study examined whether mesenchymal stem cells (MSCs), which are stem cells originated from embryonic mesoderm, are able to differentiate into functional hepatocyte-like cells in vitro. MSCs were isolated from human bone marrow and umbilical cord blood, and the surface phenotype and the mesodermal multilineage differentiation potentials of these cells were characterized and tested. To effectively induce hepatic differentiation, we designed a novel 2-step protocol with the use of hepatocyte growth factor and oncostatin M. After 4 weeks of induction, cuboidal morphology, which is characteristic of hepatocytes, was observed, and cells also expressed marker genes specific of liver cells in a time-dependent manner. Differentiated cells further demonstrated in vitro functions characteristic of liver cells, including albumin production, glycogen storage, urea secretion, uptake of low-density lipoprotein, and phenobarbital-inducible cytochrome P450 activity. In conclusion, human MSCs from different sources are able to differentiate into functional hepatocyte-like cells and, hence, may serve as a cell source for tissue engineering and cell therapy of hepatic tissues. Furthermore, the broad differentiation potential of MSCs indicates that a revision of the definition may be required.
    Keywords: Gastroenterology & Hepatology
    Date: 2004-12
    Relation: Hepatology. 2004 Dec;40(6):1275-1284.
    Link to: http://dx.doi.org/10.1002/hep.20469
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-9139&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000225583800008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644283018
    Appears in Collections:[彭汪嘉康(1996-2007)] 期刊論文
    [林景太(1999-2007)] 期刊論文
    [陳俊叡(2003-2007)] 期刊論文
    [李冠德(1997-2004)] 期刊論文

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