國家衛生研究院 NHRI:Item 3990099045/2952

NHRI > National Institute of Cancer Research > Jacqueline Whang-Peng(1996-2007) > Periodical Articles > Item 3990099045/2952

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2952

Title:	In vitro hepatic differentiation of human mesenchymal stem cells
Authors:	Lee, KD;Kuo, TKC;Whang-Peng, J;Chung, YF;Lin, CT;Chou, SH;Chen, JR;Chen, YP;Lee, OKS
Contributors:	National Institute of Cancer Research
Abstract:	This study examined whether mesenchymal stem cells (MSCs), which are stem cells originated from embryonic mesoderm, are able to differentiate into functional hepatocyte-like cells in vitro. MSCs were isolated from human bone marrow and umbilical cord blood, and the surface phenotype and the mesodermal multilineage differentiation potentials of these cells were characterized and tested. To effectively induce hepatic differentiation, we designed a novel 2-step protocol with the use of hepatocyte growth factor and oncostatin M. After 4 weeks of induction, cuboidal morphology, which is characteristic of hepatocytes, was observed, and cells also expressed marker genes specific of liver cells in a time-dependent manner. Differentiated cells further demonstrated in vitro functions characteristic of liver cells, including albumin production, glycogen storage, urea secretion, uptake of low-density lipoprotein, and phenobarbital-inducible cytochrome P450 activity. In conclusion, human MSCs from different sources are able to differentiate into functional hepatocyte-like cells and, hence, may serve as a cell source for tissue engineering and cell therapy of hepatic tissues. Furthermore, the broad differentiation potential of MSCs indicates that a revision of the definition may be required.
Keywords:	Gastroenterology & Hepatology
Date:	2004-12
Relation:	Hepatology. 2004 Dec;40(6):1275-1284.
Link to:	http://dx.doi.org/10.1002/hep.20469
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-9139&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000225583800008
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644283018
Appears in Collections:	[Jacqueline Whang-Peng(1996-2007)] Periodical Articles [Ching-Tai Lin(1999-2007)] Periodical Articles [Jim-Ray Chen(2003-2007)] Periodical Articles [Kuan-Te Li(1997-2004)] Periodical Articles

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