國家衛生研究院 NHRI:Item 3990099045/2847
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    题名: A novel EBNA-1 tag system for high level expression and efficient detection of fusion proteins in vitro and in vivo
    作者: Chen, MR;Huang, HW;Fen, JY;Chen, JY
    贡献者: National Institute of Cancer Research
    摘要: A highly specific monoclonal antibody, which recognises an epitope between amino acids (aa) 408 and 446 of EBNA-1, was found to immunoprecipitate this protein with great efficiency. By adding a 39-aa tag, the BGLF4 protein product of EBV was shown to express in the cytoplasm of transfected cells. This EBNA-1 tag could be used for the detection of specific protein expression by immunoblotting, immunofluorescence and, especially, immunoprecipitation assays. A plasmid vector encoding this EBNA-1 tag sequence was therefore designed for efficient expression both in vitro and in vivo. The efficient translational start signal of black beetle virus (BBV) was placed under the control of the SV40 or T7 promoters, and the beta-globin splicing signal used to enhance the transportation of mRNA from the nucleus to the cytoplasm. (C) 2000 Elsevier Science B.V. All rights reserved.
    关键词: Biochemical Research Methods;Biotechnology & Applied Microbiology;Virology
    日期: 2000-03
    關聯: Journal of Virological Methods. 2000 Mar;85(1-2):35-41.
    Link to: http://dx.doi.org/10.1016/S0166-0934(99)00148-2
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-0934&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000085701900004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033960390
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