國家衛生研究院 NHRI:Item 3990099045/2847
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2847


    Title: A novel EBNA-1 tag system for high level expression and efficient detection of fusion proteins in vitro and in vivo
    Authors: Chen, MR;Huang, HW;Fen, JY;Chen, JY
    Contributors: National Institute of Cancer Research
    Abstract: A highly specific monoclonal antibody, which recognises an epitope between amino acids (aa) 408 and 446 of EBNA-1, was found to immunoprecipitate this protein with great efficiency. By adding a 39-aa tag, the BGLF4 protein product of EBV was shown to express in the cytoplasm of transfected cells. This EBNA-1 tag could be used for the detection of specific protein expression by immunoblotting, immunofluorescence and, especially, immunoprecipitation assays. A plasmid vector encoding this EBNA-1 tag sequence was therefore designed for efficient expression both in vitro and in vivo. The efficient translational start signal of black beetle virus (BBV) was placed under the control of the SV40 or T7 promoters, and the beta-globin splicing signal used to enhance the transportation of mRNA from the nucleus to the cytoplasm. (C) 2000 Elsevier Science B.V. All rights reserved.
    Keywords: Biochemical Research Methods;Biotechnology & Applied Microbiology;Virology
    Date: 2000-03
    Relation: Journal of Virological Methods. 2000 Mar;85(1-2):35-41.
    Link to: http://dx.doi.org/10.1016/S0166-0934(99)00148-2
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-0934&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000085701900004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033960390
    Appears in Collections:[Jen-Yang Chen] Periodical Articles

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