國家衛生研究院 NHRI:Item 3990099045/2485
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    题名: Quantitative measurement of male steroid hormones using automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry and comparison with radioimmunoassay
    作者: Chang, YC;Li, CM;Li, LA;Jong, SB;Liao, PC;Chang, LW
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: A specific and sensitive method using high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) equipped with automatic on-line solid-phase extraction device for the quantitative measurement of anabolic hormone residues, 4-androstene-3,17-dione, testosterone and dihydrotestosterone in cell culture medium was developed. Steroid content in cell culture medium was determined directly without an additional sample preparation step. Separation of analytes from polar endogenous compounds was carried out on an automatic column-switching device coupled with a C4-alkyl-diol silica restricted-access solid-phase extraction column. The lipophilic fraction containing anabolic hormone residues were back-flushed on to a conventional C-18 reversed-phase column for the final chromatography. The analyte was ionized in an ElectroSpray interface under positive ion mode before entering a quadrupole mass analyzer. The lowest points of calibration curves were 0.05 ng ml(-1) for 4-androstene-3,17-dione and testosterone, and 1 ng ml(-1) dihydrotestosterone, respectively. A comparison with results from radioimmunoassay (RIA) is also presented.
    关键词: Chemistry, Analytical
    日期: 2003
    關聯: Analyst. 2003;128(4):363-368.
    Link to: http://dx.doi.org/10.1039/b210111b
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0003-2654&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000182064900012
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0242668444
    显示于类别:[張惠華(1999-2009)] 期刊論文
    [張玉珍(2000-2003)] 期刊論文
    [李立安] 期刊論文

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