國家衛生研究院 NHRI:Item 3990099045/2485

NHRI > National Institute of Environmental Health Sciences > Louis W. Chang(1999-2009) > Periodical Articles > Item 3990099045/2485

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2485

Title:	Quantitative measurement of male steroid hormones using automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry and comparison with radioimmunoassay
Authors:	Chang, YC;Li, CM;Li, LA;Jong, SB;Liao, PC;Chang, LW
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	A specific and sensitive method using high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) equipped with automatic on-line solid-phase extraction device for the quantitative measurement of anabolic hormone residues, 4-androstene-3,17-dione, testosterone and dihydrotestosterone in cell culture medium was developed. Steroid content in cell culture medium was determined directly without an additional sample preparation step. Separation of analytes from polar endogenous compounds was carried out on an automatic column-switching device coupled with a C4-alkyl-diol silica restricted-access solid-phase extraction column. The lipophilic fraction containing anabolic hormone residues were back-flushed on to a conventional C-18 reversed-phase column for the final chromatography. The analyte was ionized in an ElectroSpray interface under positive ion mode before entering a quadrupole mass analyzer. The lowest points of calibration curves were 0.05 ng ml(-1) for 4-androstene-3,17-dione and testosterone, and 1 ng ml(-1) dihydrotestosterone, respectively. A comparison with results from radioimmunoassay (RIA) is also presented.
Keywords:	Chemistry, Analytical
Date:	2003
Relation:	Analyst. 2003;128(4):363-368.
Link to:	http://dx.doi.org/10.1039/b210111b
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0003-2654&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000182064900012
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0242668444
Appears in Collections:	[Louis W. Chang(1999-2009)] Periodical Articles [Yu-Chen Chang(2000-2003)] Periodical Articles [Lih-Ann Li] Periodical Articles

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