國家衛生研究院 NHRI:Item 3990099045/1910
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    題名: Diversity of SHV and TEM beta-lactamases in Klebsiella pneumoniae: Gene evolution in northern Taiwan and two novel beta-lactamases, SHV-25 and SHV-26
    作者: Chang, FY;Siu, LK;Fung, CP;Huang, MH;Ho, M
    貢獻者: Division of Clinical Research
    摘要: A total of 113 blood culture isolates of Klebsiella pneumoniae from 10 hospitals in northern Taiwan were studied for SHV and TEM beta -lactamase production. bla(SHV) was amplified from all isolates by PCR. TEM-type resistance, was found in 32 of the isolates and was of the TEM-I type in all isolates. SHV-1, -2, -5, -11, and -12 and two novel enzymes were identified. These novel enzymes were designated SHV-25 and SHV-26 and had pis of 7.5 and 7.6, respectively. Amino acid differences in comparison to the amino acid sequence of bla(SHV-1) were found at positions T18A (ThrACC --> AlaGCC), L35Q (LeuCTA --> GluCAA), and M129V (MetATG --> ValGTG) for SHIV-25 and at position A187T (AlaGCC --> ThrACC) for SHV-26. The results of substrate profiles and MIC determinations showed that the novel enzymes did not hydrolyze extended-spectrum cephalosporins, rendering the isolates susceptible to these agents. Inhibition profiles revealed that the 50% inhibitory concentration for SHV-26 was higher than those for SHV-1 and SHV-25, resulting in an intermediate resistance to amoxicillin-clavulanic acid. Forty-nine ribotypes were identified, suggesting that major clonal spread had not occurred in any of the hospitals. According to the amino acid sequence, SHV beta -lactamases in Taiwan may basically be derived through stepwise mutation from SHV-1 or SHV-11 and further subdivided by four routes. The stepwise mutations initiated from SHV-1 or SHV-11 to SHV-2, SHV-5, and SHV-12 comprise the evolutionary change responsible for extended-spectrum beta -lactamase (ESBL) production in Taiwan. The stepwise mutations that lead to a non-ESBL (SHV-25) and the beta -lactamase (SHV-26) with reduced susceptibility to clavulanic acid are possibly derived from SHV-11 and SHV-1, respectively. The results suggest a stepwise evolution of SHV beta -lactamases in Taiwan.
    關鍵詞: Microbiology;Pharmacology & Pharmacy
    日期: 2001-09
    關聯: Antimicrobial Agents and Chemotherapy. 2001 Sep;45(9):2407-2413.
    Link to: http://dx.doi.org/10.1128/AAC.45.9.2407-2413.2001
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000170588500001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034878806
    顯示於類別:[何曼德(1997-2002)] 期刊論文
    [蕭樑基] 期刊論文

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