國家衛生研究院 NHRI:Item 3990099045/1910

NHRI > National Institute of Infectious Diseases and Vaccinology > Monto Ho(1997-2002) > Periodical Articles > Item 3990099045/1910

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Title:	Diversity of SHV and TEM beta-lactamases in Klebsiella pneumoniae: Gene evolution in northern Taiwan and two novel beta-lactamases, SHV-25 and SHV-26
Authors:	Chang, FY;Siu, LK;Fung, CP;Huang, MH;Ho, M
Contributors:	Division of Clinical Research
Abstract:	A total of 113 blood culture isolates of Klebsiella pneumoniae from 10 hospitals in northern Taiwan were studied for SHV and TEM beta -lactamase production. bla(SHV) was amplified from all isolates by PCR. TEM-type resistance, was found in 32 of the isolates and was of the TEM-I type in all isolates. SHV-1, -2, -5, -11, and -12 and two novel enzymes were identified. These novel enzymes were designated SHV-25 and SHV-26 and had pis of 7.5 and 7.6, respectively. Amino acid differences in comparison to the amino acid sequence of bla(SHV-1) were found at positions T18A (ThrACC --> AlaGCC), L35Q (LeuCTA --> GluCAA), and M129V (MetATG --> ValGTG) for SHIV-25 and at position A187T (AlaGCC --> ThrACC) for SHV-26. The results of substrate profiles and MIC determinations showed that the novel enzymes did not hydrolyze extended-spectrum cephalosporins, rendering the isolates susceptible to these agents. Inhibition profiles revealed that the 50% inhibitory concentration for SHV-26 was higher than those for SHV-1 and SHV-25, resulting in an intermediate resistance to amoxicillin-clavulanic acid. Forty-nine ribotypes were identified, suggesting that major clonal spread had not occurred in any of the hospitals. According to the amino acid sequence, SHV beta -lactamases in Taiwan may basically be derived through stepwise mutation from SHV-1 or SHV-11 and further subdivided by four routes. The stepwise mutations initiated from SHV-1 or SHV-11 to SHV-2, SHV-5, and SHV-12 comprise the evolutionary change responsible for extended-spectrum beta -lactamase (ESBL) production in Taiwan. The stepwise mutations that lead to a non-ESBL (SHV-25) and the beta -lactamase (SHV-26) with reduced susceptibility to clavulanic acid are possibly derived from SHV-11 and SHV-1, respectively. The results suggest a stepwise evolution of SHV beta -lactamases in Taiwan.
Keywords:	Microbiology;Pharmacology & Pharmacy
Date:	2001-09
Relation:	Antimicrobial Agents and Chemotherapy. 2001 Sep;45(9):2407-2413.
Link to:	http://dx.doi.org/10.1128/AAC.45.9.2407-2413.2001
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000170588500001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034878806
Appears in Collections:	[Monto Ho(1997-2002)] Periodical Articles [Leung-Kei Siu] Periodical Articles

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