國家衛生研究院 NHRI:Item 3990099045/16206
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    題名: The hanging-heart chip: A portable microfluidic device for high-throughput generation of contractile embryonic stem cell-derived cardiac spheroids
    作者: Lai, PT;He, CK;Li, CH;Matahum, J;Tang, CY;Hsu, CH
    貢獻者: Institute of Biomedical Engineering and Nanomedicine
    摘要: Stem cell-derived cardiac spheroids are promising models for cardiac research and drug testing. However, generating contracting cardiac spheroids remains challenging because of the laborious experimental procedure. Here, we present a microfluidic hanging-heart chip (HH-chip) that uses a microchannel and flow-driven system to facilitate cell loading and culture medium replacement operations to reduce the laborious manual handling involved in the generation of a large quantity of cardiac spheroids. The effectiveness of the HH-chip was demonstrated by simultaneously forming 50 mouse embryonic stem cell-derived embryonic bodies, which sequentially differentiated into 90% beating cardiac spheroids within 15 days of culture on the chip. A comparison of our HH-chip method with traditional hanging-drop and low-attachment plate methods revealed that the HH-chip could generate higher contracting proportions of cardiac spheroids with higher expression of cardiac markers. Additionally, we verified that the contraction frequencies of the cardiac spheroids generated from the HH-chip were sensitive to cardiotoxic drugs. Overall, our results suggest that the microfluidic hanging drop chip-based approach is a high-throughput and highly efficient method for generating contracting mouse embryonic stem cell-derived cardiac spheroids for cardiac toxicity and drug testing applications.
    日期: 2024-10-08
    關聯: Bioengineering and Translational Medicine. 2024 Oct 08;Article in Press.
    Link to: http://dx.doi.org/10.1002/btm2.10726
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001330577100001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85205913549
    顯示於類別:[許佳賢] 期刊論文

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