國家衛生研究院 NHRI:Item 3990099045/16206

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/16206

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/16206

Title:	The hanging-heart chip: A portable microfluidic device for high-throughput generation of contractile embryonic stem cell-derived cardiac spheroids
Authors:	Lai, PT;He, CK;Li, CH;Matahum, J;Tang, CY;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Stem cell-derived cardiac spheroids are promising models for cardiac research and drug testing. However, generating contracting cardiac spheroids remains challenging because of the laborious experimental procedure. Here, we present a microfluidic hanging-heart chip (HH-chip) that uses a microchannel and flow-driven system to facilitate cell loading and culture medium replacement operations to reduce the laborious manual handling involved in the generation of a large quantity of cardiac spheroids. The effectiveness of the HH-chip was demonstrated by simultaneously forming 50 mouse embryonic stem cell-derived embryonic bodies, which sequentially differentiated into 90% beating cardiac spheroids within 15 days of culture on the chip. A comparison of our HH-chip method with traditional hanging-drop and low-attachment plate methods revealed that the HH-chip could generate higher contracting proportions of cardiac spheroids with higher expression of cardiac markers. Additionally, we verified that the contraction frequencies of the cardiac spheroids generated from the HH-chip were sensitive to cardiotoxic drugs. Overall, our results suggest that the microfluidic hanging drop chip-based approach is a high-throughput and highly efficient method for generating contracting mouse embryonic stem cell-derived cardiac spheroids for cardiac toxicity and drug testing applications.
Date:	2024-10-08
Relation:	Bioengineering and Translational Medicine. 2024 Oct 08;Article in Press.
Link to:	http://dx.doi.org/10.1002/btm2.10726
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001330577100001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85205913549
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

Files in This Item:

File	Description	Size	Format
ISI001330577100001.pdf		5114Kb	Adobe PDF	8	View/Open

Loading...