國家衛生研究院 NHRI:Item 3990099045/13531

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/13531

Title:	In vivo assessment of hypoxia levels in pancreatic tumors using a dual-modality ultrasound/photoacoustic imaging system
Authors:	Wang, Y;Jhang, DF;Tsai, CH;Chiang, NJ;Tsao, CH;Chuang, CC;Chen, LT;Wayne Chang, WS;Liao, LD
Contributors:	Institute of Biomedical Engineering and Nanomedicine;National Institute of Cancer Research
Abstract:	Noninvasive anatomical and functional imaging has become an essential tool to evaluate tissue oxygen saturation dynamics in preclinical or clinical studies of hypoxia. Our dual-wavelength technique for photoacoustic (PA) imaging based on the differential absorbance spectrum of oxyhe-moglobin (oxy-Hb) and deoxyhemoglobin (deoxy-Hb) can quantify tissue oxygen saturation using the intrinsic contrast property. PA imaging of tissue oxygen saturation can be used to monitor tumor-related hypoxia, which is a particularly relevant functional parameter of the tumor microenvironment that has a strong influence on tumor aggressiveness. The simultaneous acquisition of anatomical and functional information using dual-modality ultrasound (US) and PA imaging technology enhances the preclinical applicability of the method. Here, the developed dual-modality US/PA system was used to measure relative tissue oxygenation using the dual-wavelength technique. Tissue oxygen saturation was quantified in a pancreatic tumor mouse model. The differences in tissue oxygenation were detected by comparing pancreatic samples from normal and tumor-bearing mice at various time points after implantation. The use of an in vivo pancreatic tumor model revealed changes in hypoxia at various stages of tumor growth. The US/PA imaging data positively correlated with the results of immunohistochemical staining for hypoxia. Thus, our dual-modality US/PA imaging system can be used to reliably assess and monitor hypoxia in pancreatic tumor mouse models. These findings enable the use of a combination of US and PA imaging to acquire anatomical and functional information on tumor growth and to evaluate treatment responses in longitudinal preclinical studies.
Date:	2021-06-07
Relation:	Micromachines. 2021 Jun 7;12(6):Article number 668.
Link to:	http://dx.doi.org/10.3390/mi12060668
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2072-666X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000666952500001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85108351956
Appears in Collections:	[Lun-De Liao] Periodical Articles [Wun-Shaing Wayne Chang] Periodical Articles [Li-Tzong Chen] Periodical Articles [Nai-Jung Chiang] Periodical Articles

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