Androgen receptor (AR), an androgen-activated transcription factor, plays essential roles in the development, progression, and metastasis of prostate cancer (PCa). Previously, we reported that in the absence of androgen, re-expression of AR in PC-3 cells suppresses cell migration and invasion. In this study, we determine the effect of AR on PCa cell invasion under androgen treatment. Surprisingly, androgen stimulates migration and invasion of PC-3 cells re-expressing AR. Micro-Western Array analysis indicates that androgen increases Slug, Snail, Twist, N-cadherin and YAP, and decrease E-cadherin in PC-3 cell re-expressing AR. Co-IP assay also showed AR interacts with YAP in this PCa cell. Our finding suggests that AR has opposite regulatory role on PCa invasion in the absence vs. presence of androgen.
