國家衛生研究院 NHRI:Item 3990099045/10788
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    题名: High-throughput formation and long-term culture of embryoid bodies using a microfluidic hanging drop chip
    作者: Wu, HW;Yao, WY;Hsiao, YH;Hsu, CH
    贡献者: Institute of Biomedical Engineering and Nanomedicine
    摘要: In this paper, we present a microfluidic chip-based method for high-Throughput formation and long-Term culture of embryoid bodies (EBs). The microfluidic chip is designed to be a stand-Alone device whose operation can be performed by a manual pipette, making it easy to use in a regular biology laboratory setup. We show that uniform-sized mouse EBs can be formed after 1 day of culture in the microfluidic hanging drop chip. By exchanging the medium in the chip once every 2 days during culture, the EBs could be cultured on chip for up to 7 days. The immunostaining result of the normal germ layer formation of the EBs after the 7 days culture, showing the potential of our microfluidic chip to be a useful tool for long-Term induction of EB differentiation.
    日期: 2017-08-29
    關聯: 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. 2017 Aug 29:351-354.
    Link to: http://dx.doi.org/10.1109/NEMS.2017.8017040
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000425214800198
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