國家衛生研究院 NHRI:Item 3990099045/10788

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Title:	High-throughput formation and long-term culture of embryoid bodies using a microfluidic hanging drop chip
Authors:	Wu, HW;Yao, WY;Hsiao, YH;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In this paper, we present a microfluidic chip-based method for high-Throughput formation and long-Term culture of embryoid bodies (EBs). The microfluidic chip is designed to be a stand-Alone device whose operation can be performed by a manual pipette, making it easy to use in a regular biology laboratory setup. We show that uniform-sized mouse EBs can be formed after 1 day of culture in the microfluidic hanging drop chip. By exchanging the medium in the chip once every 2 days during culture, the EBs could be cultured on chip for up to 7 days. The immunostaining result of the normal germ layer formation of the EBs after the 7 days culture, showing the potential of our microfluidic chip to be a useful tool for long-Term induction of EB differentiation.
Date:	2017-08-29
Relation:	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. 2017 Aug 29:351-354.
Link to:	http://dx.doi.org/10.1109/NEMS.2017.8017040
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000425214800198
Appears in Collections:	[Chia-Hsien Hsu] Conference Papers/Meeting Abstract

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