國家衛生研究院 NHRI:Item 3990099045/9872
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    題名: A PDMS-based microfluidic hanging drop chip for embryoid body formation
    作者: Wu, HW;Hsiao, YH;Chen, CC;Yet, SF;Hsu, CH
    貢獻者: Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
    摘要: The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 x 12 opening wells in PDMS supported by a glass substrate. The PDMS channels were fabricated by replicating polydimethyl-siloxane (PDMS) from SU-8 mold. The droplet formation in the chip was tested with different hydrostatic pressures to obtain optimal operation pressures for the wells with 1000 mum diameter openings. The droplets formed at the opening wells were used to culture mouse embryonic stem cells which could subsequently developed into EBs in the hanging droplets. This device also allows for medium exchange of the hanging droplets making it possible to perform immunochemistry staining and characterize EBs on chip.
    日期: 2016-07-06
    關聯: Molecules. 2016 Jul 6;21(7):Article number 882.
    Link to: http://dx.doi.org/10.3390/molecules21070882
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1420-3049&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000381509700067
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84979036042
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