國家衛生研究院 NHRI:Item 3990099045/9655

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Title:	Enzyme-free dissociation of neurospheres by a microfluidic chip-based method
Authors:	Lin, CH;Chang, HC;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
Abstract:	Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80-85 %), single-cell yield (91-95 %), and recovery (75-93 %).
Date:	2016-04-05
Relation:	Methods in Molecular Biology. 2016 Apr 5;1516:289-297.
Link to:	http://dx.doi.org/10.1007/7651_2016_348
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles [Ing-Ming Chiu] Periodical Articles

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