國家衛生研究院 NHRI:Item 3990099045/9609

NHRI > National Institute of Infectious Diseases and Vaccinology > Joe Yen-Hung Chow > Periodical Articles > Item 3990099045/9609

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/9609

Title:	Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture
Authors:	Shao, HY;Hsu, HS;Yu, SL;Wu, SR;Hu, KC;Chang, CK;Liu, CC;Chow, YH
Contributors:	Division of Vaccine Research and Development
Abstract:	We have developed an efficient cell culture process to scale up the production of a recombinant adenovirus that expresses the membrane-trunked fusion protein of respiratory syncytial virus (RSV; Ad-F0DeltaTM). Adherent cells of human embryonic kidney (HEK) 293-derived cell, 293A, which supports the production of E1/E3-deleted Ad-F0DeltaTM when cultured in the presence of fetal bovine serum (FBS), were adapted to suspension growth under serum-free medium. In doing so, we studied the immunogenicity of Ad-F0DeltaTMsus, which propagated in a bioreactor that was cultured with serum-free suspension of 293A, in comparison with Ad-F0DeltaTMadh, which was produced from parental 293A cells that were adherently cultured in medium containing FBS. The size and morphology of Ad-F0DeltaTMsus and Ad-F0DeltaTMadh virions were identical upon inspection with electron microscopy. The results showed that anti-F IgG and RSV-neutralizing titer were raised in the serum of both mice that were intranasally immunized twice with Ad-F0DeltaTMsus or Ad-F0DeltaTMadh at two-week injection intervals. Furthermore, the immune responses persisted for six months after vaccination. Activation of F protein-specific CD8+ T cell's epitope associated IFN- and IL-4 was induced in both Ad-F0DeltaTMsus- and Ad-F0DeltaTMadh, but not in Ad-LacZsus, -immunized mouse splenocytes. No vaccine-enhanced lung inflammation, airway mucus occlusionor eosinophils infiltration were observed in Ad-immunized mice followed by RSV challenge; however, these symptoms were observed following immunization with formalin-inactivated RSV vaccine. These results indicate that the safety and potency of Ad-F0DeltaTM produced from either adherent cells or suspension and serum-free cells are the same.
Date:	2016-06
Relation:	Antiviral Research. 2016 Jun;130:27-35.
Link to:	http://dx.doi.org/10.1016/j.antiviral.2016.03.011
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0166-3542&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000376217600004
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84962278321
Appears in Collections:	[Joe Yen-Hung Chow] Periodical Articles [Chia-Chyi Liu] Periodical Articles

Files in This Item:

File	Description	Size	Format
PUB27001351.pdf		2075Kb	Adobe PDF	539	View/Open

Loading...