國家衛生研究院 NHRI:Item 3990099045/9443

NHRI > Institute of Biomedical Engineering and Nanomedicine > Feng-Huei Lin > Periodical Articles > Item 3990099045/9443

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/9443

Title:	MWCNT-Fe3O4-based immuno-PCR for the early screening of nasopharyngeal carcinoma
Authors:	Liu, CC;Subramaniam, S;Savitha, S;Lin, FH
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Nasopharyngeal carcinoma (NPC) is the most prevalent form of malignancy in southeast China and its development is meticulously related to EBV pathogenesis. The current screening techniques are unsatisfactory in terms of the sensitivity and hence most of the NPC patients are diagnosed at an advanced stage. Herein, we report the multi-walled carbon nanotubes (MWCNTs) combined with iron oxide nanoparticles as a sensing surface for the early screening of nasopharyngeal carcinoma (NPC) by immuno-PCR (iPCR). The MWCNT-Fe3O4 nanocomposite was characterized by Fourier transform infrared spectra (FTIR), Raman spectra, X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). The characterization techniques had confirmed the successful formation of MWCNT-Fe3O4 nanocomposites. The MWCNT-Fe3O4-based iPCR was effectively tested for the quantification of anti-EBV antibodies in human serum and the limit of detection (LOD) was compared with ELISA. The limit of detection by iPCR was valid until 1:10,000,000 fold dilution of NPC+ ve human serum, whereas ELISA can detect the anti-EBV antibodies in human serum up to 1:100,000 fold dilution. The MWCNT-Fe3O4 offers an excellent surface area for the antigen-antibody binding and hence greater sensitivity was achieved.
Date:	2016-04
Relation:	Materials Science and Engineering: C. 2016 Apr;61:422-428.
Link to:	http://dx.doi.org/10.1016/j.msec.2015.12.055
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0928-4931&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000370303600052
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84953302112
Appears in Collections:	[Feng-Huei Lin] Periodical Articles

Files in This Item:

File	Description	Size	Format
SDO0928493115306664.pdf		854Kb	Adobe PDF	362	View/Open

Loading...