國家衛生研究院 NHRI:Item 3990099045/9389

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Title:	Nanoparticle-engineered probing of neural stem cells targeted therapy for glioblastoma
Authors:	Cheng, SH;Balyasnikova, I;Tsai, HM;Lai, XC;Meng, LJ;Aboody, K;Lesniak, M;Lo, LW;Chen, CT
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Objectives: Nanoparticles have been applied as efficient radioisotope carriers. The goal of this work is to (i) label/encapsulate 111In with a high concentration in the mesoporous silica nanoparticles (MSNs) for the purpose of signal amplification and to (ii) optimize loading of stem cells with 111In-MSNs for improved contrast of stem cells-based tracking system for glioblastoma. Methods: MSNs were synthesized using the sol-gel method and modified the 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) as a chelator to incorporate 111In. A new ultrahigh-resolution stationary SPECT system was utilized, which is based on the 2nd generation energy-resolved photon-counting (ERPC) CdTe detectors, offering an intrinsic resolution of 350μm and an excellent energy resolution of 3-4kev. Results: The labeling efficacy of MSNs with 111In was optimized to 95% corresponding to 695 μCi per mg of MSNs. To load the stem cells with radiolabeled MSNs, 3×105 neural stem cells (NSCs, HB1.F3.CD) were incubated with 0.2 mg/mL MSN-DOTA-111In for 2 hrs. The uptake efficacy of MSN-DOTA-111In by NSCs was 68.2%. No cytotoxicity of MSN-DOTA-111In on NSCs was observed in 2hr post-incubation as quantified by MTT assay. To quantify the NSCs using the new SPECT system, we loaded the phantom with 1, 5, 30, 300×103 NSCs corresponding to 0.5, 2.5, 15, 150 μCi of 111In activity, respectively. The activity as low as 0.5 μCi, equivalent to 1,000 NSCs, was observed using the new SPECT system in a 40-min scan. Preliminary animal studies also demonstrated similar performance for in vivo imaging. Conclusions: We have demonstrated the effective uptake of the 111In-labeled MSNs by NSCs, leading to improved SPECT image contrast in cell tracking. SPECT imaging of nanoparticle-engineered NSCs can be evaluated for their targeting efficacy in the animal model of glioblastoma.
Date:	2014-05
Relation:	Journal of Nuclear Medicine. 2014 May;55(Suppl. 1):Abstract number 1042.
Link to:	http://jnm.snmjournals.org/content/55/supplement_1/1042
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0161-5505&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000361438101206
Appears in Collections:	[Leu-Wei Lo] Conference Papers/Meeting Abstract

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