Loading...
|
Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/9194
|
| Title: | Rho-associated kinase inhibitors promote the cardiac differentiation of embryonic and induced pluripotent stem cells |
| Authors: | Cheng, YT;Yeih, DF;Liang, SM;Chien, CY;Yu, YL;Ko, BS;Jan, YJ;Kuo, CC;Sung, LY;Shyue, SK;Chen, MF;Yet, SF;Wu, KK;Liou, JY |
| Contributors: | Institute of Cellular and Systems Medicine |
| Abstract: | AbstractBackground Rho-associated kinase (ROCK) plays an important role in maintaining embryonic stem (ES) cell pluripotency. To determine whether ROCK is involved in ES cell differentiation into cardiac and hematopoietic lineages, we evaluated the effect of ROCK inhibitors, Y-27632 and fasudil on murine ES and induced pluripotent stem (iPS) cell differentiation. Methods Gene expression levels were determined by real-time PCR, Western blot analysis and immunofluorescent confocal microscopy. Cell transplantation of induced differentiated cells were assessed in vivo in a mouse model (three groups, n = 8/group) of acute myocardial infarction (MI). The cell engraftment was examined by immunohistochemical staining and the outcome was analyzed by echocardiography. Results Cells were cultured in hematopoietic differentiation medium in the presence or absence of ROCK inhibitor and colony formation as well as markers of ES, hematopoietic stem cells (HSC) and cells of cardiac lineages were analyzed. ROCK inhibition resulted in a drastic change in colony morphology accompanied by loss of hematopoietic markers (GATA-1, CD41 and β-Major) and expressed markers of cardiac lineages (GATA-4, Isl-1, Tbx-5, Tbx-20, MLC-2a, MLC-2v, α-MHC, cTnI and cTnT) in murine ES and iPS cells. Fasudil-induced cardiac progenitor (Mesp-1 expressing) cells were infused into a murine MI model. They engrafted into the peri-infarct and infarct regions and preserved left ventricular function. Conclusions These findings provide new insights into the signaling required for ES cell differentiation into hematopoietic as well as cardiac lineages and suggest that ROCK inhibitors are useful in directing iPS cell differentiation into cardiac progenitor cells for cell therapy of cardiovascular diseases. |
| Date: | 2015-12 |
| Relation: | International Journal of Cardiology. 2015 Dec;201:441-448. |
| Link to: | http://dx.doi.org/10.1016/j.ijcard.2015.08.118 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0167-5273&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000366144200127 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84943528174 |
| Appears in Collections: | [劉俊揚] 期刊論文
[伍焜玉] 期刊論文
[林秀芳] 期刊論文
[郭呈欽] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| SDO0167527315303478.pdf | | 2187Kb | Adobe PDF | 611 | View/Open |
|
All items in NHRI are protected by copyright, with all rights reserved.
|
