國家衛生研究院 NHRI:Item 3990099045/9141
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 913492      線上人數 : 1130
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/9141


    題名: Growth arrest DNA damage-inducible gene 45 gamma expression as a prognostic and predictive biomarker in hepatocellular carcinoma
    其他題名: Growth arrest DNA damage-inducible gene 45 γ expression as a prognostic and predictive biomarker in hepatocellular carcinoma
    作者: Ou, DL;Shyue, SK;Lin, LI;Feng, ZR;Liou, JY;Fan, HH;Lee, BS;Hsu, C;Cheng, AL
    貢獻者: Institute of Cellular and Systems Medicine
    摘要: Growth arrest DNA damage-inducible gene 45 (GADD45) family proteins play a crucial role in regulating cellular stress responses and apoptosis. The present study explored the prognostic and predictive role of GADD45gamma in hepatocellular carcinoma (HCC) treatment. GADD45gamma expression in HCC cells was examined using quantitative reverse transcription-PCR (qRT-PCR) and Western blotting. The control of GADD45gamma transcription was examined using a luciferase reporter assay and chromatin immunoprecipitation. The in vivo induction of GADD45gamma was performed using adenoviral transfer. The expression of GADD45gamma in HCC tumor tissues from patients who had undergone curative resection was measured using qRT-PCR. Sorafenib induced expression of GADD45gamma mRNA and protein, independent of its RAF kinase inhibitor activity. GADD45gamma induction was more prominent in sorafenib-sensitive HCC cells (Huh-7 and HepG2, IC50 6-7 muM) than in sorafenib-resistant HCC cells (Hep3B, Huh-7R, and HepG2R, IC50 12-15 muM). Overexpression of GADD45gamma reversed sorafenib resistance in vitro and in vivo, whereas GADD45gamma expression knockdown by using siRNA partially abrogated the proapoptotic effects of sorafenib on sorafenib-sensitive cells. Overexpression of survivin in HCC cells abolished the antitumor enhancement between GADD45gamma overexpression and sorafenib treatment, suggesting that survivin is a crucial mediator of antitumor effects of GADD45gamma. GADD45gamma expression decreased in tumors from patients with HCC who had undergone curative surgery, and low GADD45gamma expression was an independent prognostic factor for poor survival, in addition to old age and vascular invasion. The preceding data indicate that GADD45gamma suppression is a poor prognostic factor in patients with HCC and may help predict sorafenib efficacy in HCC.
    日期: 2015-09
    關聯: Oncotarget. 2015 Sep;6(29):27953-27965.
    Link to: http://dx.doi.org/10.18632/oncotarget.4446
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000363161300112
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84942330447
    顯示於類別:[劉俊揚] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB26172295.pdf6364KbAdobe PDF468檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋