本發明係描述可供重組蛋白表現的組合物及方法,其係可應用於廣泛範圍之細胞類型。本發明組合物包含一段選自果蠅labial (lab)基因之IRES序列,或其變異序列或片段;抑或是lab基因之IRES序列之同源序列、同源序列之變異序列或片段。此外,還揭示了這些組合物的使用方法。The invention describes compositions and methods for recombinant protein expression in a wide range of cell types. The compositions comprise an IRES sequence from the Drosophila labial (lab) gene, or a variant or fragment thereof, or alternatively, a homolog of a lab IRES, or a variant or fragment thereof. Methods of using the compositions are also described.