本發明係關於一種包含德國麻疹病毒(RV)融合基因之嵌合型病毒粒子。該RV融合基因包含第一核苷酸序列，其編碼缺乏RV E1蛋白之RV蛋白，及第二核苷酸序列，其以符合轉譯框架之方式與該第一核苷酸序列連接，且編碼產生體液性免疫的病毒蛋白。該嵌合型病毒粒子不含RV E1蛋白-編碼基因。本發明亦揭示一種生產包含有RV融合基因之嵌合型病毒粒子的病毒包裝細胞，以及包含以符合轉譯框架之方式與啟動子連接之RV融合基因的單離表現載體。A chimeric viral particle that comprises a RV fusion gene is disclosed. The RV fusion gene comprises a first nucleotide sequence encoding a RV that is devoid of RV E1 protein, and a second nucleotide sequence that linked in translation frame to the first nucleotide sequence and encodes a humoral immunogenic viral protein. The chimeric viral particle is free of RV E1 protein-encoding gene. A virus packaging cell that generates the chimeric viral particle comprising a RV fusion gene and an isolated expression vector comprising a RV fusion gene linked in translation frame to a promoter are also disclosed.
