國家衛生研究院 NHRI:Item 3990099045/8864

NHRI > Institute of Cellular and Systems Medicine > Yasuhisa Fukui(2010-2016) > Periodical Articles > Item 3990099045/8864

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8864

Title:	SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts
Authors:	Chang, YT;Shu, CL;Lai, JY;Ching-Yu, L;Chuu, CP;Morishita, K;Ichikawa, T;Jessberger, R;Fukui, Y
Contributors:	Institute of Cellular and Systems Medicine
Abstract:	Mouse embryo fibroblasts (MEFs) grow slowly after cultivation from animals, however, after an extended period of cultivation, their growth accelerates. We found that SWAP-70 deficient MEFs failed to increase growth rates. They maintain normal growth rates and proliferation cycles for at least 5 years. Complementing SWAP-70 deficiency in one of these MEF clones, MEF1F2, by expressing human SWAP-70 resulted in fast growth of the cells after further cultivation for a long period. The resulting cells show a transformation phenotype, since they grow on top of each other and do not show contact inhibition. This phenotype was reverted when sanguinarine, a putative SWAP-70 inhibitor, was added. Two SWAP-70 expressing clones were examined in detail. Even after cell density became very high their cdc2 and NFκB were still activated suggesting that they do not stop growing. One of the clones formed colonies in soft agar and formed tumors in nude mice. Lately, one more clone became transformed being able to make colonies in soft agar. We maintain 4 human SWAP-70 expressing MEF1F2 cell lines. Three out of 4 clones exhibited transforming phenotypes. The mouse SWAP-70 gene also promoted transformation of MEFs. Taken together our data suggest that SWAP-70 is not a typical oncogene, but is required for spontaneous transformation of MEFs.
Date:	2015-07
Relation:	Experimental Cell Research. 2015 Jul;345(2):150-157.
Link to:	http://dx.doi.org/10.1016/j.yexcr.2015.06.011
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0014-4827&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000380179600005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84990036827
Appears in Collections:	[Yasuhisa Fukui(2010-2016)] Periodical Articles [Chih-Pin Chuu] Periodical Articles

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