國家衛生研究院 NHRI:Item 3990099045/8850
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    题名: The effect of red light and far-red light conditions on secondary metabolism in Agarwood
    作者: Kuo, TCY;Chen, CH;Chen, SH;Lu, IH;Chu, MJ;Huang, LC;Lin, CY;Chen, CY;Lo, HF;Jeng, ST;Chen, LFO
    贡献者: Division of Biostatistics and Bioinformatics
    摘要: Background: Agarwood, a heartwood derived from Aquilaria trees, is a valuable commodity that has seen prevalent use among many cultures. In particular, it is widely used in herbal medicine and many compounds in agarwood are known to exhibit medicinal properties. Although there exists much research into medicinal herbs and extraction of high value compounds, few have focused on increasing the quantity of target compounds through stimulation of its related pathways in this species. Results: In this study, we observed that cucurbitacin yield can be increased through the use of different light conditions to stimulate related pathways and conducted three types of high-throughput sequencing experiments in order to study the effect of light conditions on secondary metabolism in agarwood. We constructed genome-wide profiles of RNA expression, small RNA, and DNA methylation under red light and far-red light conditions. With these profiles, we identified a set of small RNA which potentially regulates gene expression via the RNA-directed DNA methylation pathway. Conclusions: We demonstrate that light conditions can be used to stimulate pathways related to secondary metabolism, increasing the yield of cucurbitacins. The genome-wide expression and methylation profiles from our study provide insight into the effect of light on gene expression for secondary metabolism in agarwood and provide compelling new candidates towards the study of functional secondary metabolic components.
    日期: 2015-06-12
    關聯: BMC Plant Biology. 2015 Jun 12;15:Article number 139.
    Link to: http://dx.doi.org/10.1186/s12870-015-0537-y
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1471-2229&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000355969200001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84931033377
    显示于类别:[林仲彥(1999-2005)] 期刊論文

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