國家衛生研究院 NHRI:Item 3990099045/8659

NHRI > Institute of Molecular and Genomic Medicine > Yi-Rong Chen > Periodical Articles > Item 3990099045/8659

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8659

Title:	Akt suppresses DLK for maintaining self-renewal of mouse embryonic stem cells
Authors:	Wu, CC;Wu, HJ;Wang, CH;Lin, CH;Hsu, SC;Chen, YR;Hsiao, M;Schuyler, SC;Leigh Lu, F;Ma, N;Lu, J
Contributors:	Institute of Molecular and Genomic Medicine;Division of Vaccine Research and Development
Abstract:	Mouse embryonic stem cells (ES cells) can proliferate indefinitely. To identify potential signals involved in suppression of self-renewal, we previously screened a kinase/phosphatase expression library in ES cells, and observed that inhibition of Dual Leucine zipper-bearing Kinase (DLK) increased relative cell numbers. DLK protein was detected in both the pluripotent and differentiated states of mouse ES cells while DLK kinase activity increased upon differentiation. Overexpression of DLK in mouse ES cells displayed reductions in relative cell/colony numbers and Nanog expression, suggesting a suppressive role of DLK in self-renewal. By examining protein sequences of DLK, we identified 2 putative Akt phosphorylation sites at S584 and T659. Blocking PI3K/Akt signaling with LY-294002 enhanced DLK kinase activity dramatically. We found that Akt interacts with and phosphorylates DLK. Mutations of DLK amino acid residues at putative Akt phosphorylation sites (S584A, T659A, or S584A and T659A) diminished the level of DLK phosphorylation. While the mutated DLKs (S584A, T659A, or S584A and T659A) were expressed, a further reduction in cell/colony numbers and Nanog expression appeared in mouse ES cells. In addition, these mutant DLKs (S584A, T659A, or S584A and T659A) exhibited more robust kinase activity and cell death compared to wild type DLK or green fluorescence (GFP) controls. In summary, our results show that DLK functions to suppress self-renewal of mouse ES cells and is restrained by Akt phosphorylation.
Date:	2015-04-18
Relation:	Cell Cycle. 2015 Apr 18;14(8):1207-1217.
Link to:	http://dx.doi.org/10.1080/15384101.2015.1014144
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1538-4101&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000353168600016
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84928013407
Appears in Collections:	[Yi-Rong Chen] Periodical Articles [Shu-Ching Hsu] Periodical Articles

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