Loading...
|
Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/8623
|
| Title: | The virulence effect caused by wzi gene in Klebsiella pneumoniae |
| Authors: | Huang, LY;Chen, FH;Chang, JC;Tsai, YK;Liou, CH;Ma, L;Lin, FM;Ding, YJ;Chen, PJ;Lin, JC;Yeh, KM;Siu, LK |
| Contributors: | Division of Infectious Diseases |
| Abstract: | Purpose: Klebsiella pneumoniae (Kp) is a well-known pathogen and a common reason of community-acquired and nosocomial infections causing liver abscesses, endophthalmitis, or meningitis. Capsular polysaccharide (CPS) is one of the most important virulent factors in Kp. The virulence effect caused by wzi gene that is involved in the attachment of the repeat unit of CPS to outer membrane remains uncertain. Previous studies indicate that deduced Wzi protein sequence is corresponding to capsular serotype (K-type). This study aimed to examine the gene actual effect on virulence and its changes associated with K-type switch. Methods: In-frame deletion and replacement was used to obtain the deletion and switched mutants of the wzi gene in Kp strain 312, which isolated from liver abscess patient and harbored K20 CPS cluster. The wzi gene of switched mutant was replaced from K20-type to K1-type. The virulence was assessed through serum killing, neutrophil phagocytosis and mouse lethality assay. Results: Comparison to the parental 312 strain (K20, serum-sensitive, phagocytosis-resistant, LD50=102 cfu), 312Δ wzi mutant decreased 102-fold of LD50 and exhibited more susceptible in serum killing and phagocytosis. LD50 of 312Δ wzi::K1-wzi mutant was 103 cfu and there was no significant difference in virulence assay between 312Δ wzi and 312Δ wzi::K1-wzi strains. Conclusions: The study demonstrates that wzi gene has impact in virulence in mice model, and contributes to serum killing and phagocytosis of Kp strains. Furthermore, replacement of distinct K-type wzi gene can also influence the mice lethality. |
| Date: | 2015-04 |
| Relation: | Journal of Microbiology, Immunology and Infection. 2015 Apr;48(2, Suppl.1):S132. |
| Link to: | http://dx.doi.org/10.1016/j.jmii.2015.02.464 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1684-1182&DestApp=IC2JCR |
| Appears in Collections: | [蕭樑基] 會議論文/會議摘要
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| SDO1684118215004971.pdf | | 49Kb | Adobe PDF | 552 | View/Open |
|
All items in NHRI are protected by copyright, with all rights reserved.
|
