國家衛生研究院 NHRI:Item 3990099045/8622
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 921633      線上人數 : 1447
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/8622


    題名: Regulation of AcrAB-TolC and the roles of this efflux pump on antibiotic resistance and virulence in Klebsiella pneumoniae
    作者: Tsai, YK;Chang, JC;Liou, CH;Hsiao, YW;Ma, L;Huang, LY;Lin, FM;Ding, YJ;Chen, PJ;Siu, LK
    貢獻者: Division of Infectious Diseases
    摘要: Purpose: The regulation mechanism of the AcrAB-TolC efflux pump in Klebsiella pneumoniae remains unclear. This study clarified this mechanism and further evaluated the roles of this efflux pump on antibiotic resistance and virulence in K. pneumoniae. Methods: In-frame deletion mutagenesis was used to generate the deletions of the acrB, tolC and the potential regulator genes of the AcrAB-TolC (acrR, ramR, marR, soxR and rob) in K. pneumoniae strains HK1269S and NVT2001S, which belong to capsular serotype K1 and K2, respectively. The gene expression levels and antibiotic susceptibility was determined, while the virulence was assessed by in vitro and in vivo studies. Results: In both of the two K. pneumoniae strains, the deletion of acrR, marR, soxR or rob showed slight effects on the expression levels of acrB, tolC and ramA, while the deletion of ramR significantly increased these expressions. Compared to their parental strains, the ΔramR strains showed a significant (≥4-fold) increase in the MICs of multiple antibiotics, and the ΔacrB and ΔtolC strains exhibited a significant (≥4-fold) decrease, while no significant differences were found in the ΔacrR, ΔmarR, ΔsoxR and Δrob strains. Compared with the serum-resistance strain NVT2001S, its ΔramR strain became susceptible to serum, and ∼5.7- and ∼11.7-fold smaller survival rates (P<0.05) in serum were found in its ΔacrB and ΔtolC strains, respectively. The deletion of ramR also caused a ∼8.7-fold smaller survival rate (P<0.05) in serum in the serum-susceptible strain HK1269S. In a mouse peritonitis model, the ΔacrB strains showed no change in virulence, while and ΔramR and ΔtolC strains presented significantly (P<0.05) lower virulence. Conclusions: The AcrAB-TolC efflux pump in K. pneumoniae is mainly regulated by the ramR and ramA genes. The ramR deletion in K. pneumoniae confers the overexpression of AcrAB-TolC and results in the antibiotic-resistance increase and virulence reduction at the same time.
    日期: 2015-04
    關聯: Journal of Microbiology, Immunology and Infection. 2015 Apr;48(2, Suppl.1):S173-S174.
    Link to: http://dx.doi.org/10.1016/j.jmii.2015.02.608
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1684-1182&DestApp=IC2JCR
    顯示於類別:[蕭樑基] 會議論文/會議摘要

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    SDO1684118215006416.pdf367KbAdobe PDF526檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋