國家衛生研究院 NHRI:Item 3990099045/8403
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 925375      線上人數 : 805
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/8403


    題名: Methamphetamine induces a rapid increase of intracellular Ca++ levels in neurons overexpressing GCaMP5
    作者: Yu, SJ;Wu, KJ;Bae, EK;Hsu, MJ;Richie, CT;Harvey, BK;Wang, Y
    貢獻者: Center for Neuropsychiatric Research
    摘要: In this study, methamphetamine (Meth)- and glutamate (Glu)-mediated intracellular Ca++ (Ca++ i) signals were examined in real time in primary cortical neurons overexpressing an intracellular Ca++ probe, GCaMP5, by adeno-associated viral (AAV) serotype 1. Binding of Ca++ to GCaMP increased green fluorescence intensity in cells. Both Meth and Glu induced a rapid increase in Ca++ i, which was blocked by MK801, suggesting that Meth enhanced Ca++ i through Glu receptor in neurons. The Meth-mediated Ca++ signal was also blocked by Mg++ , low Ca++ or the L-type Ca++ channel inhibitor nifedipine. The ryanodine receptor inhibitor dantrolene did not alter the initial Ca++ influx but partially reduced the peak of Ca++ i. These data suggest that Meth enhanced Ca++ influx through membrane Ca++ channels, which then triggered the release of Ca++ from the endoplasmic reticulum in the cytosol. AAV-GCaMP5 was also injected to the parietal cortex of adult rats. Administration of Meth enhanced fluorescence in the ipsilateral cortex. Using immunohistochemistry, Meth-induced green fluorescence was found in the NeuN-containing cells in the cortex, suggesting that Meth increased Ca++ in neurons in vivo. In conclusion, we have used in vitro and in vivo techniques to demonstrate a rapid increase of Ca++ i by Meth in cortical neurons through overexpression of GCaMP5. As Meth induces behavioral responses and neurotoxicity through Ca++ i, modulation of Ca++ i may be useful to reduce Meth-related reactions.
    日期: 2016-03
    關聯: Addiction Biology. 2016 Mar;21(2):255-266.
    Link to: http://dx.doi.org/10.1111/adb.12193
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1355-6215&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000371148700004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84959343991
    顯示於類別:[王昀] 期刊論文
    [劉誠珍] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB25377775.pdf1065KbAdobe PDF667檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋