國家衛生研究院 NHRI:Item 3990099045/8386

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/8386

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8386

Title:	Electronic hydroxyl radical microsensors based on the conductivity change of polyaniline
Authors:	Fang, JY;Chu, CH;Sarang, I;Fang, KC;Hsu, CP;Huang, YF;Hsu, CH;Chen, CC;Li, SS;Yeh, AJ;Yao, DJ;Wang, YL
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	In this study, conductive polyaniline (PANI) was coated on Si3N4/Si substrates and reacted with hydroxyl radicals created by mixing ferrous ions and hydrogen peroxide in a buffer solution, as known as the Fenton reaction. The hydroxyl radicals reacted with PANI, resulting in the decreased conductivity of PANI. The reduction in the conductivity of PANI was found to be proportional to the concentration of hydroxyl radicals in the buffer solution. The concentration of hydroxyl radicals was calibrated by comparing the fluorescence intensities of the Amplex ultrared molecules oxidized by radicals and hydrogen peroxide via the catalysis of horseradish peroxidase (HRP), respectively. The conductivity change of the PANI shows a linear relationship to the concentration of hydroxyl radicals, ranging from 0.2 μM to 0.8 μM. The conductivity of PANI does not respond to either ferrous ions or hydrogen peroxide alone. Only when both ferrous ions and hydrogen peroxide are present in the solution, the conductivity of PANI decreases, indicating that the conductivity change of PANI is attributed to the existence of hydroxyl radicals. The simple process for the sensor fabrication allows the sensor to be cost-effective and disposable. This electronic sensor can detect one of the most important reactive oxygen species (ROS), the hydroxyl radical, and therefore is promising for studying oxidative stress.
Date:	2015-03
Relation:	Sensors and Actuators B: Chemical. 2015 Mar;208:99-105.
Link to:	http://dx.doi.org/10.1016/j.snb.2014.11.016
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0925-4005&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000346000500014
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84911908788
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles

Files in This Item:

File	Description	Size	Format
SDO0925400514013781.pdf		1611Kb	Adobe PDF	582	View/Open

Loading...