國家衛生研究院 NHRI:Item 3990099045/8319
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    題名: IL-1beta promotes malignant transformation and tumor aggressiveness in oral cancer
    其他題名: IL-1β promotes malignant transformation and tumor aggressiveness in oral cancer
    作者: Lee, CH;Chang, JS;Syu, SH;Wong, TS;Chan, JY;Tang, YC;Yang, ZP;Yang, WC;Chen, CT;Lu, SC;Tang, PH;Yang, TC;Chu, PY;Hsiao, JR;Liu, KJ
    貢獻者: National Institute of Cancer Research;Institute of Biotechnology and Pharmaceutical Research
    摘要: Chronic inflammation, coupled with alcohol, betel quid, and cigarette consumption, is associated with oral squamous cell carcinoma (OSCC). Interleukin-1 beta (IL-1beta) is a critical mediator of chronic inflammation and implicated in many cancers. In this study, we showed that increased pro-IL-1beta expression was associated with the severity of oral malignant transformation in a mouse OSCC model induced by 4-Nitroquinolin-1-oxide (4-NQO) and arecoline, two carcinogens related to tobacco and betel quid, respectively. Using microarray and quantitative PCR assay, we showed that pro-IL-1beta was upregulated in human OSCC tumors associated with tobacco and betel quid consumption. In a human OSCC cell line TW2.6, we demonstrated nicotine-derived nitrosamine ketone (NNK) and arecoline stimulated IL-1beta secretion in an inflammasome-dependent manner. IL-1beta treatment significantly increased the proliferation and dysregulated the Akt signaling pathways of dysplastic oral keratinocytes (DOKs). Using cytokine antibodies and inflammation cytometric bead arrays, we found that DOK and OSCC cells secreted high levels of IL-6, IL-8, and growth-regulated oncogene-alpha following IL-1beta stimulation. The conditioned medium of IL-1beta-treated OSCC cells exerted significant proangiogenic effects. Crucially, IL-1beta increased the invasiveness of OSCC cells through the epithelial-mesenchymal transition (EMT), characterized by downregulation of E-cadherin, upregulation of Snail, Slug, and Vimentin, and alterations in morphology. These findings provide novel insights into the mechanism underlying OSCC tumorigenesis. Our study suggested that IL-1beta square can be induced by tobacco and betel quid-related carcinogens, and participate in the early and late stages of oral carcinogenesis by increasing the proliferation of dysplasia oral cells, stimulating oncogenic cytokines, and promoting aggressiveness of OSCC. J. Cell. Physiol.
    日期: 2015-04
    關聯: Journal of Cellular Physiology. 2015 APR;230(4):875-884.
    Link to: http://dx.doi.org/10.1002/jcp.24816
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-9541&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000346916900016
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84919772748
    顯示於類別:[劉柯俊] 期刊論文
    [張書銘] 期刊論文
    [李家惠] 期刊論文
    [陳炯東] 期刊論文

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