國家衛生研究院 NHRI:Item 3990099045/8116
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    題名: A poorly neutralizing IgG2a/c response elicited by a DNA vaccine protects mice against Japanese encephalitis virus
    作者: Chen, HW;Huang, HW;Hu, HM;Chung, HH;Wu, SH;Chong, P;Tao, MH;Pan, CH
    貢獻者: Division of Vaccine Research and Development
    摘要: We previously demonstrated that immunization with a DNA vaccine expressing the JEV envelope (E) protein conferred a high level of protection through a poorly neutralizing antibody response. In this report, we further investigated the role of IgG subclass in this antibody-dependent protection using cytokine co-immunization and cytokine-deficient mice. A significant difference in IgG2a/c but not IgG1 was observed between mice that survived or died following a lethal challenge. Correspondingly, the IgG2a/c response and protection increased in IL-4-deficient mice but decreased in IFN-gamma-deficient mice, highlights the importance of IgG2a/c. In addition, the restoration of protection and E-specific IgG2a/c production in IFN-gamma-deficient mice by a Th1-biased intramuscular immunization suggests that IgG2a/c but not IFN-gamma is the major component for protection. The failure of protection against a direct intracranial challenge indicated that IgG2a/c-mediated protection is restricted to outside the CNS. Consistent with this conclusion, passive transfer of E-specific antisera conferred protection only pre-exposure to JEV. Therefore, our data here provide evidence that IgG subclass plays an important role in the protection against JEV, particular in poorly neutralizing E-specific antibodies; and Th1-biased IgG2a/c confers a better protection than Th2-biased IgG1 to against JEV.
    日期: 2014-09
    關聯: Journal of General Virology. 2014 Sep;95(Pt 9):1983-1990.
    Link to: http://dx.doi.org/10.1099/vir.0.067280-0
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-1317&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000341800500013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84906268551
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