國家衛生研究院 NHRI:Item 3990099045/8084
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8084


    Title: Functional effects of TGF-beta 1 on mesenchymal stem cell mobilization in cockroach allergen-induced asthma
    Other Titles: Functional effects of TGF-β 1 on mesenchymal stem cell mobilization in cockroach allergen-induced asthma
    Authors: Gao, PS;Zhou, YF;Xian, LL;Li, CJ;Xu, T;Plunkett, B;Huang, SK;Wan, M;Cao, X
    Contributors: Division of Environmental Health and Occupational Medicine
    Abstract: Mesenchymal stem cells (MSCs) have been suggested to participate in immune regulation and airway repair/remodeling. TGF-beta 1 is critical in the recruitment of stem/progenitor cells for tissue repair, remodeling, and cell differentiation. In this study, we sought to investigate the role of TGF-beta 1 in MSC migration in allergic asthma. We examined nestin expression (a marker for MSCs) and TGF-beta 1 signaling activation in airways in cockroach allergen extract (CRE)-induced mouse models. Compared with control mice, there were increased nestin(+) cells in airways and higher levels of active TGF-b1 in serum and p-Smad2/3 expression in lungs of CRE-treated mice. Increased activation of TGF-beta 1 signaling was also found in CRE-treated MSCs. We then assessed MSC migration induced by conditioned medium from CRE-challenged human epithelium in air/liquid interface culture in Transwell assays. MSC migration was stimulated by epithelial-conditioned medium, but was significantly inhibited by either TGF-beta 1-neutralizing Ab or T beta R1 inhibitor. Intriguingly, increased migration of MSCs from blood and bone marrow to the airway was also observed after systemic injection of GFP(+) MSCs and from bone marrow of Nes-GFP mice following CRE challenge. Furthermore, TGF-beta 1-neutralizing Ab inhibited the CRE-induced MSC recruitment, but promoted airway inflammation. Finally, we investigated the role of MSCs in modulating CRE-induced T cell response and found that MSCs significantly inhibited CRE-induced inflammatory cytokine secretion (IL-4, IL-13, IL-17, and IFN-g) by CD4(+) T cells. These results suggest that TGF-beta 1 may be a key promigratory factor in recruiting MSCs to the airways in mouse models of asthma.
    Date: 2014-05
    Relation: Journal of Immunology. 2014 May;192(10):4560-4570.
    Link to: http://dx.doi.org/10.4049/jimmunol.1303461
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-1767&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000335973600013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84901261243
    Appears in Collections:[Shau-Ku Huang] Periodical Articles

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