國家衛生研究院 NHRI:Item 3990099045/8048

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Title:	Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
Authors:	De Beer, JL;Ködm̈on, C;Van Ingen, J;Supply, P;Van Soolingen, D;Jamieson, FB;Bidovec-Stojkovic, U;Brown, T;Cirillo, DM;Cruz, L;Miranda, A;Dou, HY;Fauville-Dufaux, M;Fitzgibbon, MM;García De Viedma, D;Groenheit, R;Haanperä-Heikkinen, M;Indra, A;Kam, KM;Kramer, R;Jiang, GL;Niemann, S;Obrovac, M;Rasmussen, EM;Refrégier, G;Realpe, T;Samper, S;Sharma, MK;Sougakoff, W;Stakenas, P;Stavrum, R;Trenkler, J;Wada, T;Siame, KK;Tafaj, S;Cowan, L;Sng, LH;Seagar, AL;Basu, I;Rastogi, N;Ferro, BE;De Matos, F;Kipnis, A
Contributors:	Division of Infectious Diseases
Abstract:	BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing. OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries. RESULTS: The inter- and intra-laboratory reproducibility increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci. CONCLUS ION: This second worldwide proficiency study indicates a substantial increase in the reproducibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex.
Date:	2014-05-01
Relation:	International Journal of Tuberculosis and Lung Disease. 2014 May 1;18(5):594-600.
Link to:	http://dx.doi.org/10.5588/ijtld.13.0531
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1027-3719&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000335627800018
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84896486105
Appears in Collections:	[Horng-Yunn Dou] Periodical Articles

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