國家衛生研究院 NHRI:Item 3990099045/7625
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/7625


    Title: SitA contributes to the virulence of Klebsiella pneumoniae in a mouse infection model
    Authors: Sun, WSW;Syu, W, Jr.;Ho, WL;Lin, CN;Tsai, SF;Wang, SH
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Klebsiella pneumoniae is an opportunistic pathogen, which causes a wide range of nosocomial infections. Recently, antibiotic resistance makes K. pneumoniae infection difficult to deal with. Investigation on virulence determinants of K. pneumoniae can provide more information about pathogenesis and unveil new targets for treatment or vaccine development. In this study, SitA, a Fur-regulated divalent cation transporter, was found significantly increased when K. pneumoniae was cultured in a nutrient-limited condition. A sitA-deletion strain (ΔsitA) was created to characterize the importance of SitA in virulence. ΔsitA showed higher sensitivity toward hydroperoxide than its parental strain. In a mouse intraperitoneal infection model, the survival rate of mice infected with ΔsitA strain increased greatly when compared with that of mice infected with the parental strain, suggesting that sitA deletion attenuates the bacterial virulence in vivo. To test whether ΔsitA strain is a potential vaccine candidate, mice were immunized with inactivated bacteria and then challenged with the wild-type strain. The results showed that using ΔsitA mutant protected mice better than using the wild-type strain or the capsule-negative congenic bacteria. In summary, SitA was found being important for the growth of K. pneumoniae in vivo and deleting sitA might be a potential approach to generate vaccines against K. pneumoniae.
    Date: 2014-02
    Relation: Microbes and Infection. 2014 Feb;16(2):161-170.
    Link to: http://dx.doi.org/10.1016/j.micinf.2013.10.019
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1286-4579&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000347866300009
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84894999839
    Appears in Collections:[Shih-Feng Tsai] Periodical Articles

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