國家衛生研究院 NHRI:Item 3990099045/7428

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chung-Shi Yang > Periodical Articles > Item 3990099045/7428

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/7428

Title:	Enhanced cell growth and tumorigenicity of rat glioma cells by stable expression of human CD133 through multiple molecular actions
Authors:	Fang, KM;Lin, TC;Chan, TC;Ma, SZ;Tzou, BC;Chang, WR;Liu, JJ;Chiou, SH;Yang, CS;Tzeng, SF
Contributors:	Center for Nanomedicine Research
Abstract:	CD133 (Prominin-1/AC133) is generally treated as a cell surface marker found on multipotent stem cells and tumor stem-like cells, and its biological function remains debated. Genetically modified rat glioma cell lines were generated by lentiviral gene delivery of human CD133 into rat C6 glioma cells (hCD133(+)-C6) or by infection of C6 cells with control lentivirus (mock-C6). Stable hCD133 expression promoted the self-renewal ability of C6-formed spheres with an increase in the expression of the stemness markers, Bmi-1 and SOX2. Akt phosphorylation, Notch-1 activation, and Notch-1 target gene expression (Hes-1, Hey1 and Hey2) were increased in hCD133(+)-C6 when compared to mock-C6. (+)-C6 cells effectively suppressed their clonogenic ability, indicating that these factors are involved in expanding the growth of hCD133(+)-C6. An elevated expression of G (+)-C6. A decline in the invasion of hCD133(+)-C6 by knockdown of Arhgap27 expression indicated the critical role of Arhgap27 in promoting cell migration of hCD133(+)-C6. In vivo study further showed that hCD133(+)-C6 formed aggressive tumors in vivo compared to mock-C6. Exposure of hCD133(+)-C6 to arsenic trioxide not only reduced Akt phosphorylation, Notch-1 activation and Hes-1 expression in vitro, but also inhibited their tumorigenicity in vivo. (+)-C6 in vitro, as well as progressive tumor formation in vivo.
Date:	2013-09
Relation:	Glia. 2013 Sep;61(9):1402-1417.
Link to:	http://dx.doi.org/10.1002/glia.22521
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0894-1491&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000322331700002
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84880771285
Appears in Collections:	[Chung-Shi Yang] Periodical Articles

Files in This Item:

File	Description	Size	Format
ISI000322331700002.pdf		1658Kb	Adobe PDF	575	View/Open

Loading...