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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/7407
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| Title: | A gene expression signature of epithelial tubulogenesis and a role for ASPM in pancreatic tumor progression |
| Authors: | Wang, WY;Hsu, CC;Wang, TY;Li, CR;Hou, YC;Chu, JM;Lee, CT;Liu, MS;Su, JJ;Jian, KY;Huang, SS;Jiang, SS;Shan, YS;Lin, PW;Shen, YY;Lee, MT;Chan, TS;Chang, CC;Chen, CH;Chang, IS;Lee, YL;Chen, LT;Tsai, KK |
| Contributors: | National Institute of Cancer Research;Pathology Core Laboratory |
| Abstract: | BACKGROUND: & Aims: Many patients with pancreatic ductal adenocarcinoma (PDAC) develop recurrent or metastatic diseases following surgery, so it is important to identify those most likely to benefit from aggressive therapy. Disruption of tissue microarchitecture is an early step in pancreatic tumorigenesis and a parameter used in pathology grading of glandular tumors. We investigated whether changes in gene expression during pancreatic epithelial morphogenesis were associated with outcomes of patients with PDAC after surgery. METHODS: We generated architectures of human pancreatic duct epithelial (HPDE) cells in a 3-dimensional basement membrane matrix. We identified gene expression profiles of the cells during different stages of tubular morphogenesis (tubulogenesis) and of PANC-1 cells during spheroid formation. Differential expression of genes was confirmed by immunoblot analysis. We compared the gene expression profile associated with pancreatic epithelial tubulogenesis with that of PDAC samples from 27 patients, as well as with their outcomes after surgery. RESULTS: We identified a gene expression profile associated with tubulogenesis that resembled the profile of human pancreatic tissue with differentiated morphology and exocrine function. Patients with PDACs with this profile fared well following surgery. Based on this profile, we established a 6-28 gene tubulogenesis-specific signature that accurately determined the prognosis of independent cohorts of patients with PDAC (total n = 128; accuracy = 81.2%-95.0%). One gene ASPM, was downregulated during tubulogenesis, but upregulated in human PDAC cell lines and tumor samples; upregulation correlated with patient outcome (Cox regression P = .0028). Bioinformatic, genetic, biochemical, functional, and clinical correlative studies showed that ASPM promotes aggressiveness of PDAC by maintaining Wnt-beta-catenin signaling and stem cell features of PDAC cells. CONCLUSIONS: We identified a gene expression profile associated with pancreatic epithelial tubulogenesis and a tissue architecture-specific signature of PDAC cells that is associated with patient outcome following surgery. |
| Date: | 2013-11 |
| Relation: | Gastroenterology. 2013 Nov;145(5):1110-1120. |
| Link to: | http://dx.doi.org/10.1053/j.gastro.2013.07.040 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0016-5085&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000325977600037 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84886775070 |
| Appears in Collections: | [蔡坤志] 期刊論文
[陳立宗] 期刊論文
[江士昇] 期刊論文
[張憶壽] 期刊論文
[沈瑩瑩] 期刊論文
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| PUB23896173.pdf | | 2795Kb | Adobe PDF | 758 | View/Open |
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