國家衛生研究院 NHRI:Item 3990099045/7298
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 915216      在线人数 : 1342
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7298


    题名: Transcriptional activation of FGF1 gene promoter sustains self-renewal of embryonic stem cells and neural stem cells through Aurora-A kinase signaling
    作者: Hsu, YC;Kao, CY;Liu, JW;Chung, YF;Lee, DC;Chiu, IM
    贡献者: Institute of Cellular and Systems Medicine
    摘要: Aurora-A kinase (AurA) is a centrosome and mitotic spindle- associated, cell cycle-regulated serine/threonine kinase and is a key regulator of asymmetric cell division and cell fate determination. Fibroblast growth factor 1 (FGF1) has been suggested as an important growth factor for many cell types. FGF1 and FGF-1B promoter (-540 to +31)-driven green fluorescent protein (F1BGFP) have been used to isolate neural stem/progenitor cells (NSPCs) and glioblastoma stem cells from developing mouse brains, human glioblastoma cell lines, and tissues, respectively. In this study, we provide several lines of evidence to demonstrate that AurA activation is required for self-renewal and multipotency in F1BGFP(+) stem cells: (i) Inhibition of AurA activation disrupted the maintenance of mouse embryonic stem cells (ESCs), mouse ESC-derived NSPCs, primary mouse brain NSPCs, and human glioblastoma stem cells; (ii) F1BGFP reporter could be used to isolate GFP(+) NSPCs with significantly higher levels of AurA activation, cell proliferation, and neurosphere formation; (iii) Autocrine/paracrine activation of AurA in F1BGFP(+) cells could be blocked by FGF1-neutralizing antibody, FGF receptor inhibitor SU5402, and AKT inhibitor; (iv) Inhibition of AurA activation reduced neurosphere formation of F1BGFP(+) NSPCs; (v) Markers of NSPC-multipotency also decreased upon AurA inhibition. These results suggest that AurA kinase regulates the growth and self-renewal of stem cells, such as ESCs and NSPCs. Using the F1BGFP reporter, we have provided a novel method to identify and enrich a subset of ESCs and NSPCs with AurA activation from different cellular and tissue sources.
    日期: 2013-10
    關聯: Cell Transplantation. 2013 Oct;22(5):905.
    Link to: http://dx.doi.org/10.3727/096368913X664883
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0963-6897&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000318585300047
    显示于类别:[邱英明] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000318585300047.pdf794KbAdobe PDF586检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈