國家衛生研究院 NHRI:Item 3990099045/7264
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    題名: Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice
    作者: Wang, KW;Chueh, LL;Wang, MH;Huang, YT;Fang, BH;Chang, CY;Fang, MC;Chou, JY;Hsieh, SC;Wan, CH
    貢獻者: Laboratory Animal Center
    摘要: Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (alpha-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.
    日期: 2013-04
    關聯: Laboratory Animals. 2013 Apr;47(2):116-121.
    Link to: http://dx.doi.org/10.1177/0023677213478298
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0023-6772&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000317997800006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84878079436
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