國家衛生研究院 NHRI:Item 3990099045/7207
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12189/12972 (94%)
造訪人次 : 955348      線上人數 : 683
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7207


    題名: Mechanical activation of smad, a novel regulator for endothelial cell proliferation induced by disturbed flow
    作者: Zhou, J;Lee, PL;Lee, CI;Yang, TL;Chien, S;Chiu, JJ
    貢獻者: Division of Medical Engineering Research
    摘要: Atherosclerotic lesions develop at arterial branches and curvatures, where the local flow is disturbed with low and oscillatory shear stress (OSS). We investigated the role of OSS in the endothelial cell (EC) activations of Smad1/5 and their regulation in OSS-induced EC proliferation. OSS (0.5±4 dynes/cm2) to human umbilical vein ECs induced sustained phosphorylations of Smad1/5. Smad1/5 activations were found only in the selective patches of ECs overlying foam cell lesions in human coronary arteries. These shear-activations of Smad1/5 were not inhibited by pre-treating ECs with Noggin, a specific antagonist of bone morphogenic proteins. Transfecting ECs with specific siRNA of BMPRII, BMPRIB, and v and β3 integrins inhibited OSS-induced Smad1/5 phosphorylations. OSS induced sustained increases in the association of vβ3 integrin with BMPRIB, which was inhibited by BMPRII-specific siRNA. OSS induced the activations of focal adhesion kinase/Shc/extracellular signal-regulated kinase, which are required for OSS-induced Smad1/5 phosphorylations. OSS induced increased expression of cyclin A and decreased expressions of p21CIP1 and p27KIP1 and hence proliferation in ECs. Transfecting ECs with Smad1/5-specific siRNA inhibited these OSS-induced responses in ECs. Our findings provide a molecular basis for the role of Smad1/5 in regulating EC proliferation in response to OSS.
    日期: 2010-04
    關聯: FASEB Journal. 2010 Apr;24(Suppl.):598.12.
    Link to: http://www.fasebj.org/cgi/content/meeting_abstract/24/1_MeetingAbstracts/598.12
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000208675503811
    顯示於類別:[裘正健] 會議論文/會議摘要

    文件中的檔案:

    沒有與此文件相關的檔案.



    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋