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http://ir.nhri.org.tw/handle/3990099045/7181
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| Title: | Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules |
| Authors: | Wu, JM;Chen, CT;Coumar, MS;Lin, WH;Chen, ZJ;Hsu, JT;Peng, YH;Shiao, HY;Chu, CY;Wu, JS;Lin, CT;Chen, CP;Hsueh, CC;Chang, KY;Kao, LP;Huang, CY;Chao, YS;Wu, SY;Hsieh, HP;Chi, YH |
| Contributors: | Institute of Biotechnology and Pharmaceutical Research;Institute of Cellular and Systems Medicine |
| Abstract: | The overexpression of Aurora kinases in multiple tumors makes these kinases appealing targets for the development of anticancer therapies. This study identified two small molecules with a furanopyrimidine core, IBPR001 and IBPR002, that target Aurora kinases and induce a DFG conformation change at the ATP site of Aurora A. Our results demonstrate the high potency of the IBPR compounds in reducing tumorigenesis in a colorectal cancer xenograft model in athymic nude mice. Human hepatoma up-regulated protein (HURP) is a substrate of Aurora kinase A, which plays a crucial role in the stabilization of kinetochore fibers. This study used the IBPR compounds as well as MLN8237, a proven Aurora A inhibitor, as chemical probes to investigate the molecular role of HURP in mitotic spindle formation. These compounds effectively eliminated HURP phosphorylation, thereby revealing the coexistence and continuous cycling of HURP between unphosphorylated and phosphorylated forms that are associated, respectively, with microtubules emanating from centrosomes and kinetochores. Furthermore, these compounds demonstrate a spatial hierarchical preference for HURP in the attachment of microtubules extending from the mother to the daughter centrosome. The finding of inequality in the centrosomal microtubules revealed by these small molecules provides a versatile tool for the discovery of new cell-division molecules for the development of antitumor drugs. |
| Date: | 2013-05 |
| Relation: | Proceedings of the National Academy of Sciences of the United States of America. 2013 May;110(19):E1779-E1787. |
| Link to: | http://dx.doi.org/10.1073/pnas.1220523110 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0027-8424&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000319327700011 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84877317722 |
| Appears in Collections: | [謝興邦] 期刊論文
[伍素瑩] 期刊論文
[趙宇生(2002-2013)] 期刊論文
[徐祖安] 期刊論文
[陳炯東] 期刊論文
[紀雅惠] 期刊論文
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| PUB23610398.pdf | | 2120Kb | Adobe PDF | 902 | View/Open |
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