國家衛生研究院 NHRI:Item 3990099045/7003

NHRI > Institute of Cellular and Systems Medicine > Yasuhisa Fukui(2010-2016) > Periodical Articles > Item 3990099045/7003

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Title:	Scattering of MCF7 cells by heregulin beta-1 depends on the MEK and p38 MAP kinase pathway
Other Titles:	Scattering of MCF7 cells by heregulin ß-1 depends on the MEK and p38 MAP kinase pathway
Authors:	Okoshi, R;Shu, CL;Ihara, S;Fukui, Y
Contributors:	Institute of Cellular and Systems Medicine
Abstract:	Heregulin (HRG) β1 signaling promotes scattering of MCF7 cells by inducing breakdown of adherens and tight junctions. Here, we show that stimulation with HRG-β1 causes the F-actin backbone of junctions to destabilize prior to the loss of adherent proteins and scattering of the cells. The adherent proteins dissociate and translocate from cell–cell junctions to the cytosol. Moreover, using inhibitors we show that the MEK1 pathway is required for the disappearance of F-actin from junctions and p38 MAP kinase activity is essential for scattering of the cells. Upon treatment with a p38 MAP kinase inhibitor, adherens junction complexes immediately reassemble, most likely in the cytoplasm, and move to the plasma membrane in cells dissociated by HRG-β1 stimulation. Subsequently, tight junction complexes form, most likely in the cytoplasm, and move to the plasma membrane. Thus, the p38 MAP kinase inhibitor causes a re-aggregation of scattered cells, even in the presence of HRG-β1. These results suggest that p38 MAP kinase signaling to adherens junction proteins regulates cell aggregation, providing a novel understanding of the regulation of cell–cell adhesion.
Date:	2013-01-07
Relation:	PLoS ONE. 2013 Jan 7;8(1):Article number e53298.
Link to:	http://dx.doi.org/10.1371/journal.pone.0053298
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000313429100051
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84872033569
Appears in Collections:	[Yasuhisa Fukui(2010-2016)] Periodical Articles

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