國家衛生研究院 NHRI:Item 3990099045/6908
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6908


    Title: Elimination of cancer stem-like "Side Population" cells in hepatoma cell lines by Chinese herbal mixture "Tien-Hsien Liquid"
    Authors: Yao, CJ;Yeh, CT;Lee, LM;Chuang, SE;Yeh, CF;Chao, WJ;Lai, TY;Lai, GM
    Contributors: National Institute of Cancer Research
    Abstract: There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy. We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells. After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, beta-catenin, and Oct-4 than those of non-SP cells. At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33% to 0.49%, 1.55% to 0.43%, and 1.69% to 0.27%, respectively. The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e. g., ABCG2, CD133, and SMO. The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished. Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells. Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.
    Date: 2012-11
    Relation: Evidence-Based Complementary and Alternative Medicine. 2012 Nov;2012:Artcile ID 617085.
    Link to: http://dx.doi.org/10.1155/2012/617085
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000310019500001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84868687454
    Appears in Collections:[Shuang-En Chuang] Periodical Articles
    [Gi-Ming Lai(2004-2008)] Periodical Articles

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